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The second costume, though still the same character, the costume choice sort of gives it away. Though i have known guys to wear dresses, not on a day-to-day basis thankfully, if your trying to define the character as a girl, the second two is the best.
I think it'd be awesome if you did create a comic in which you never did truly reveal the sexuality of the character. It'd be one of the those quirky comics where the reader sort of just assumes, but you never use the definitive words to label her as either one, such as "he" or "she". Give the character an ambiguous name such as Ashton, that could really make the character fall in either category. Let it have hobbies that do not have sexual barriers (no boy/girl teams). And then just when you feel like finishing series have that final punch where you identify the character's sexuality, this sort of twist can cause at least the impulse for the reader to want to read all the previous comics over.
That's my two cents
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Sometimes those who can't listen, hear more than those who do